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Home>Product>Primary Antibody

CYP19A1 Recombinant Rabbit mAb

#3269

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Recombinant Antibody
Western blotting analysis using CYP19A1 antibody (Cat#3269). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYP19A1 antibody (Cat#3269, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). CYP19A1, cytochrome P450 family 19 subfamily A member 1.
Immunocytochemical staining of HepG2 cells with CYP19A1 antibody (Cat#3269, 1:1,000). Nuclei were stained blue with DAPI; CYP19A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CYP19A1 expression in HepG2 cells using CYP19A1 antibody (Cat#3269, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red,CYP19A1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CYP19A1
  • Catalog：
    3269
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name CYP19A1 Recombinant Rabbit mAb
Aliases CYP19A1; Cytochrome P450 Family 19 Subfamily A Member; ARO; CYAR; P-450AROM; Aromatase; CYP19; CPV1; ARO; Cytochrome P450, Subfamily XIX (Aromatization Of Androgens); Cytochrome P450, Family 19, Subfamily A, Polypeptide 1; Cytochrome P-450AROM; Cytochrome P450 19A1; Estrogen Synthase; CYPXIX; Flavoprotein-Linked Monooxygenase; Microsomal Monooxygenase; Estrogen Synthetase; EC 1.14.14.14; EC 1.14.14.1; AROMATASE
Background

Gene Name: CYP19A1

NCBI Gene Entry: 1588

UniProt Entry: P11511

Application Information

Molecular Weight: Predicted, 58 kDa; observed, 58 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4800

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Aromatase
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using CYP19A1 antibody (Cat#3269). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYP19A1 antibody (Cat#3269, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). CYP19A1, cytochrome P450 family 19 subfamily A member 1.
  • Immunocytochemical staining of HepG2 cells with CYP19A1 antibody (Cat#3269, 1:1,000). Nuclei were stained blue with DAPI; CYP19A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of CYP19A1 expression in HepG2 cells using CYP19A1 antibody (Cat#3269, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red,CYP19A1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CYP19A1
  • Catalog：
    3269
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using CYP19A1 antibody (Cat#3269). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYP19A1 antibody (Cat#3269, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). CYP19A1, cytochrome P450 family 19 subfamily A member 1.
Immunocytochemical staining of HepG2 cells with CYP19A1 antibody (Cat#3269, 1:1,000). Nuclei were stained blue with DAPI; CYP19A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CYP19A1 expression in HepG2 cells using CYP19A1 antibody (Cat#3269, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red,CYP19A1.
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