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Home>Product>Primary Antibody

KD-Validated Erb-B2 Receptor Tyrosine Kinase 2 Recombinant Rabbit mAb

#64611

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using Erb-b2 receptor tyrosine kinase 2 antibody (Cat#64611). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Erb-b2 receptor tyrosine kinase 2 antibody (Cat#64611, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using erb-b2 receptor tyrosine kinase 2 antibody (Cat#64611). Erb-b2 receptor tyrosine kinase 2 expression in wild type (WT) and erb-b2 receptor tyrosine kinase 2 (ERBB2) shRNA knockdown (KD) 293T cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with erb-b2 receptor tyrosine kinase 2 antibody (Cat#64611, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Erb-b2 receptor tyrosine kinase 2 antibody (Cat#64611, 1:1,000). Nuclei were stained blue with DAPI; Erb-b2 receptor tyrosine kinase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ERBB2
  • Catalog：
    64611
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated Erb-B2 Receptor Tyrosine Kinase 2 Recombinant Rabbit mAb
Aliases Erb-B2 Receptor Tyrosine Kinase 2; HER2; NEU; C-ERB-2; C-ERB2; MLN-19; HER-2; CD340; NGL; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2; Tyrosine Kinase-Type Cell Surface Receptor HER2; Neuro/Glioblastoma Derived Oncogene Homolog; Human Epidermal Growth Factor Receptor 2; Receptor Tyrosine-Protein Kinase ErbB-2; Metastatic Lymph Node Gene 19 Protein; Proto-Oncogene C-ErbB-2; Proto-Oncogene Neu; P185(ErbB2); EC 2.7.10.1; MLN 19; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2 (Neuro/Glioblastoma Derived Oncogene Homolog); V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog 3V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncoprotein; Neuroblastoma/Glioblastoma Derived Oncogene Homolog; Metastatic Lymph Node Gene 19; C-Erb B2/Neu Protein; CD340 Antigen; P185(ERBB2); HER-2/Neu; Herstatin; P185erbB2; EC 2.7.10; VSCN2; MLN19; TKR1
Background

Gene Name: ERBB2

NCBI Gene Entry: 2064

UniProt Entry: P04626

Application Information

Molecular Weight: Predicted, 138 kDa; observed, 185 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1350

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human ErbB2 (HER2)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Erb-b2 receptor tyrosine kinase 2 antibody (Cat#64611). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Erb-b2 receptor tyrosine kinase 2 antibody (Cat#64611, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using erb-b2 receptor tyrosine kinase 2 antibody (Cat#64611). Erb-b2 receptor tyrosine kinase 2 expression in wild type (WT) and erb-b2 receptor tyrosine kinase 2 (ERBB2) shRNA knockdown (KD) 293T cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with erb-b2 receptor tyrosine kinase 2 antibody (Cat#64611, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Erb-b2 receptor tyrosine kinase 2 antibody (Cat#64611, 1:1,000). Nuclei were stained blue with DAPI; Erb-b2 receptor tyrosine kinase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ERBB2
  • Catalog：
    64611
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Erb-b2 receptor tyrosine kinase 2 antibody (Cat#64611). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Erb-b2 receptor tyrosine kinase 2 antibody (Cat#64611, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using erb-b2 receptor tyrosine kinase 2 antibody (Cat#64611). Erb-b2 receptor tyrosine kinase 2 expression in wild type (WT) and erb-b2 receptor tyrosine kinase 2 (ERBB2) shRNA knockdown (KD) 293T cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with erb-b2 receptor tyrosine kinase 2 antibody (Cat#64611, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Erb-b2 receptor tyrosine kinase 2 antibody (Cat#64611, 1:1,000). Nuclei were stained blue with DAPI; Erb-b2 receptor tyrosine kinase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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