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Home>Product>Primary Antibody

Beta-Secretase 1 Recombinant Rabbit mAb

#4326

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Recombinant Antibody
Western blotting analysis using beta-secretase 1 antibody (Cat#4326). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with beta-secretase 1 antibody (Cat#4326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Beta-secretase 1 antibody (Cat#4326, 1:1,000). Nuclei were stained blue with DAPI; Beta-secretase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Beta-secretase 1 expression in C2C12 cells using Beta-secretase 1 antibody (Cat#4326, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Beta-secretase 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    BACE1
  • Catalog：
    4326
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    M, R

Information

Data

Information
Product Name Beta-Secretase 1 Recombinant Rabbit mAb
Aliases BACE1; Beta-Secretase 1; BACE; Membrane-Associated Aspartic Protease; Beta-Site APP Cleaving Enzyme 1; Beta-Site APP-Cleaving Enzyme
Aspartyl Protease; EC 3.4.23.46; Memapsin-2; Asp 2; ASP2; Beta-Site Amyloid Beta A4 Precursor Protein-Cleaving Enzyme; Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1; Transmembrane Aspartic Proteinase Asp2; Beta-Site APP-Cleaving Enzyme 1; APP Beta-Secretase; EC 3.4.23; KIAA1149; HSPC104
Background

Gene Name: BACE1

NCBI Gene Entry: 23621

UniProt Entry: P56817

Application Information

Molecular Weight: Predicted, 56 kDa; observed, 70 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10660

Species Reactivity: Mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human BACE1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using beta-secretase 1 antibody (Cat#4326). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with beta-secretase 1 antibody (Cat#4326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with Beta-secretase 1 antibody (Cat#4326, 1:1,000). Nuclei were stained blue with DAPI; Beta-secretase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Beta-secretase 1 expression in C2C12 cells using Beta-secretase 1 antibody (Cat#4326, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Beta-secretase 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    BACE1
  • Catalog：
    4326
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    M, R
RELATED PRODUCTS
Western blotting analysis using beta-secretase 1 antibody (Cat#4326). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with beta-secretase 1 antibody (Cat#4326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Beta-secretase 1 antibody (Cat#4326, 1:1,000). Nuclei were stained blue with DAPI; Beta-secretase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Beta-secretase 1 expression in C2C12 cells using Beta-secretase 1 antibody (Cat#4326, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Beta-secretase 1.
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