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DNA Polymerase Gamma Recombinant Rabbit mAb

#4590

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Recombinant Antibody
Western blotting analysis using POLG antibody (Cat#4590). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with POLG antibody (Cat#4590, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of POLG expression in HeLa cells using POLG antibody (Cat#4590, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, POLG.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    POLG
  • Catalog:
    4590
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name DNA Polymerase Gamma Recombinant Rabbit mAb
Aliases POLG; DNA Polymerase Gamma, Catalytic Subunit; POLG1; POLGA; Mitochondrial DNA Polymerase Catalytic Subunit; Polymerase (DNA) Gamma, Catalytic Subunit; Polymerase (DNA Directed), Gamma; DNA Polymerase Subunit Gamma-1; 5'-Deoxyribose-Phosphate Lyase; 3'-5' Exodeoxyribonuclease; PolG-Alpha; EC 2.7.7.7; MDP1; Mitochondrial Polymerase Gamma Catalytic Subunit; EC 3.1.11.-; EC 4.2.99.-; MTDPS4A; MTDPS4B; MIRAS; SANDO; SCAE; PEO
Background

Gene Name: POLG

NCBI Gene Entry: 5428

UniProt Entry: P54098

Application Information

Molecular Weight: Predicted, 140 kDa; observed, 140 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11965

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human DNA Polymerase gamma
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using POLG antibody (Cat#4590). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with POLG antibody (Cat#4590, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of POLG expression in HeLa cells using POLG antibody (Cat#4590, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, POLG.
Western blotting analysis using POLG antibody (Cat#4590). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with POLG antibody (Cat#4590, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of POLG expression in HeLa cells using POLG antibody (Cat#4590, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, POLG.
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