KD-Validated Plexin A1 Recombinant Rabbit mAb - Product

KD-Validated Plexin A1 Recombinant Rabbit mAb

#62523

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Recombinant Antibody
shRNA Knockdown Validated

Western blotting analysis using plexin A1 antibody (Cat#62523). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Plexin A1 antibody (Cat#62523, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using plexin A1 antibody (Cat#62523). Plexin A1 expression in wild-type (WT) and plexin A1 (PLXNA1) shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Plexin A1 antibody (Cat#62523, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Flow cytometric analysis of plexin A1 expression in C2C12 cells using plexin A1 antibody (Cat#62523, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Plexin A1.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

PLXNA1
Catalog：

62523
Application：

WB, FCM
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	KD-Validated Plexin A1 Recombinant Rabbit mAb
Aliases	PLXNA1; Plexin A1; NOV; PLXN1; Semaphorin Receptor NOV; PLEXIN-A1; Plexin-A1; Plexin 1; DWOPNED; NOVP
Background	Gene Name: PLXNA1 NCBI Gene Entry: 5361 UniProt Entry: Q9UIW2
Application Information	Molecular Weight: Predicted, 211 kDa; observed, 240 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB1245 Species Reactivity: Human,mouse,rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human Plexin A1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using plexin A1 antibody (Cat#62523). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Plexin A1 antibody (Cat#62523, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using plexin A1 antibody (Cat#62523). Plexin A1 expression in wild-type (WT) and plexin A1 (PLXNA1) shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Plexin A1 antibody (Cat#62523, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of plexin A1 expression in C2C12 cells using plexin A1 antibody (Cat#62523, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Plexin A1.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

PLXNA1
Catalog：

62523
Application：

WB, FCM
Species Reactivity：

H, M, R

Datasheet

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