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SCARB1 Recombinant Rabbit mAb

#4107

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Recombinant Antibody
Western blotting analysis using SCARB1 antibody (Cat#4107). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SCARB1 antibody (Cat#4107, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using SCARB1 antibody (Cat#4107, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    SCARB1
  • Catalog:
    4107
  • Application:
    WB, IHC-P
  • Species Reactivity:
    H, M, R
Information
Product Name SCARB1 Recombinant Rabbit mAb
Aliases SCARB1; Scavenger Receptor Class B Member 1; CLA-1; SR-BI; SRB1; CLA1; CD36L1; CD36 Antigen (Collagen Type I Receptor, Thrombospondin Receptor)-Like 1; CD36 And LIMPII Analogous 1; Collagen Type I Receptor, Thrombospondin Receptor-Like 1; Scavenger Receptor Class B, Member 1; Scavenger Receptor Class B Type III; CD36 Antigen-Like 1; CD36 Antigen; HDLQTL6; HDLCQ6
Background

Gene Name: SCARB1

NCBI Gene Entry: 949

UniProt Entry: Q8WTV0

Application Information

Molecular Weight: Predicted, 61 kDa; observed, 75 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB9625

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)

Immunogen A synthesized peptide derived from human Scavenging Receptor SR-BI
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using SCARB1 antibody (Cat#4107). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SCARB1 antibody (Cat#4107, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using SCARB1 antibody (Cat#4107, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using SCARB1 antibody (Cat#4107). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SCARB1 antibody (Cat#4107, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using SCARB1 antibody (Cat#4107, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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