Cart

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News

Sign In/Register

Cart
  • Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products
Home>Product>Primary Antibody

KD-Validated HSPA1A Recombinant Rabbit mAb

#66887

Click to copy product information

Return to List

Recombinant Antibody
Knockdown Validated
Western blotting analysis using HSPA1A antibody (Cat#66887). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSPA1A antibody (Cat#66887, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using HSPA1A antibody (Cat#66887). HSPA1A expression in wild type (WT) and HSPA1A knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HSPA1A antibody (Cat#66887, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of Hela cells with HSPA1A (Cat#66887, 1:1,000). Nuclei were stained blue with DAPI; HSPA1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium . Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HSPA1A
  • Catalog：
    66887
  • Application：
    WB, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated HSPA1A Recombinant Rabbit mAb
Aliases HSPA1A; Heat Shock Protein Family A (Hsp70) Member 1A; HSP70-1; HSPA1; Heat Shock Protein Family A Member 1A; Heat Shock 70 KDa Protein 1A; Heat Shock 70kDa Protein 1A; Heat Shock 70 KDa Protein 1; Heat Shock 70kD Protein 1A; HSP70.1; HSP72; Epididymis Secretory Sperm Binding Protein; DnaK-Type Molecular Chaperone HSP70-1; Epididymis Secretory Protein Li 103; Heat Shock 70 KDa Protein 1A/1B; Heat Shock 70 KDa Protein 1/2; Heat Shock 70 KDa Protein 1B; Heat Shock 70 KDa Protein 2; Heat Shock-Induced Protein; HSP70-1/HSP70-2; HSP70.1/HSP70.2; HEL-S-103; HSP70-1A; HSP70-2; HSP70.2; HSP70I; HSP70; HSX70
Background

Gene Name: HSPA1A

NCBI Gene Entry: 3303

UniProt Entry: P0DMV8

Application Information

Molecular Weight: Predicted, 70 kDa; observed, 70 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB1185

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human HSPA1A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using HSPA1A antibody (Cat#66887). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSPA1A antibody (Cat#66887, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using HSPA1A antibody (Cat#66887). HSPA1A expression in wild type (WT) and HSPA1A knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HSPA1A antibody (Cat#66887, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of Hela cells with HSPA1A (Cat#66887, 1:1,000). Nuclei were stained blue with DAPI; HSPA1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium . Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HSPA1A
  • Catalog：
    66887
  • Application：
    WB, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using HSPA1A antibody (Cat#66887). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSPA1A antibody (Cat#66887, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using HSPA1A antibody (Cat#66887). HSPA1A expression in wild type (WT) and HSPA1A knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HSPA1A antibody (Cat#66887, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of Hela cells with HSPA1A (Cat#66887, 1:1,000). Nuclei were stained blue with DAPI; HSPA1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium . Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$
View cart Empty cart
  • Phone Number

    +1 (301) 545-2395

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.