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TNF Receptor II Recombinant Rabbit mAb

#7521

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Recombinant Antibody
Western blotting analysis using TNF Receptor II antibody (Cat#7521). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TNF Receptor II antibody (Cat#7521, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of TNF Receptor II expression in HT-1080 cells using TNF Receptor II antibody (Cat#7521, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TNF Receptor II.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    TNFRSF1B
  • Catalog:
    7521
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name TNF Receptor II Recombinant Rabbit mAb
Aliases TNF Receptor Superfamily Member 1B; TNFBR; P75; TNF-R-II; TNF-R75; TNFR80; CD120b; TNFR2; Tumor Necrosis Factor Receptor Superfamily Member 1B; Tumor Necrosis Factor Receptor Type II; Tumor Necrosis Factor Receptor 2; P80 TNF-Alpha Receptor; TNF-RII; TNF-R2; Tumor Necrosis Factor Receptor Superfamily, Member 1B; Tumor Necrosis Factor Binding Protein 2; Tumor Necrosis Factor Beta Receptor; P75 TNF Receptor; CD120b Antigen; Etanercept; P75TNFR; TNFR-II; TNFR1B; TBPII
Background

Gene Name: TNFRSF1B

NCBI Gene Entry: 7133

UniProt Entry: P20333

Application Information

Molecular Weight: Predicted, 48 kDa; observed, 60 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB995

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human TNF Receptor II
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using TNF Receptor II antibody (Cat#7521). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TNF Receptor II antibody (Cat#7521, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of TNF Receptor II expression in HT-1080 cells using TNF Receptor II antibody (Cat#7521, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TNF Receptor II.
Western blotting analysis using TNF Receptor II antibody (Cat#7521). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TNF Receptor II antibody (Cat#7521, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of TNF Receptor II expression in HT-1080 cells using TNF Receptor II antibody (Cat#7521, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TNF Receptor II.
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