Western blotting analysis using Hsc70 antibody (Cat#4119). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsc70 antibody (Cat#4119, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Hsc70 expression in HT-1080 cells using Hsc70 antibody (Cat#4119, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Hsc70.
HSPA8; Heat Shock Protein Family A (Hsp70) Member 8; HSC70; HSP73; HSPA10; HSC71; Lipopolysaccharide-Associated Protein 1; Heat Shock Cognate 71 KDa Protein; Heat Shock 70kDa Protein 8; LPS-Associated Protein 1; LAP-1; Epididymis Secretory Sperm Binding Protein Li 72p; N-Myristoyltransferase Inhibitor Protein 71; Constitutive Heat Shock Protein 70; Epididymis Luminal Protein 33; Heat Shock Cognate Protein 54; Heat Shock 70 KDa Protein 8; Heat Shock 70kd Protein 10; Heat Shock 70kD Protein 8; EC 3.6.4.10; HEL-S-72p; HEL-33; HSC54; HSP71; NIP71; LAP1
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human Hsc70
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using Hsc70 antibody (Cat#4119). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsc70 antibody (Cat#4119, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Hsc70 expression in HT-1080 cells using Hsc70 antibody (Cat#4119, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Hsc70.
Western blotting analysis using Hsc70 antibody (Cat#4119). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsc70 antibody (Cat#4119, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Hsc70 expression in HT-1080 cells using Hsc70 antibody (Cat#4119, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Hsc70.
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