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RNA Binding Motif Protein 8A Recombinant Rabbit mAb

#2475

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Recombinant Antibody
Western blotting analysis using RNA binding motif protein 8A antibody (Cat#2475). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RNA binding motif protein 8A antibody (Cat#2475, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with RNA binding motif protein 8A antibody (Cat#2475, 1:1,000). Nuclei were stained blue with DAPI; RNA binding motif protein 8A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of RNA binding motif protein 8A expression in HeLa cells using RNA binding motif protein 8A antibody (Cat#2475, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RNA binding motif protein 8A.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    RBM8A
  • Catalog:
    2475
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name RNA Binding Motif Protein 8A Recombinant Rabbit mAb
Aliases RNA Binding Motif Protein 8A; BOV-1A; BOV-1B; BOV-1C; ZNRP; RBM8; Y14; Exon Junction Complex Core Component Y14; RNA-Binding Protein Y14; Ribonucleoprotein RBM8A; RNA-Binding Protein 8A; Binder Of OVCA1-1; Binder Of OVCA1; BOV-1; RNA Binding Motif Protein 8B; RNA-Binding Motif Protein 8A; Ribonucleoprotein RBM8; C1DELq21.1; DEL1q21.1; MDS014; RBM8B; ZRNP1; TAR
Background

Gene Name: RBM8A

NCBI Gene Entry: 9939

UniProt Entry: Q9Y5S9

Application Information

Molecular Weight: Predicted, 20 kDa; observed, 21 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1020

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human Y14
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using RNA binding motif protein 8A antibody (Cat#2475). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RNA binding motif protein 8A antibody (Cat#2475, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of Hela cells with RNA binding motif protein 8A antibody (Cat#2475, 1:1,000). Nuclei were stained blue with DAPI; RNA binding motif protein 8A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of RNA binding motif protein 8A expression in HeLa cells using RNA binding motif protein 8A antibody (Cat#2475, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RNA binding motif protein 8A.
Western blotting analysis using RNA binding motif protein 8A antibody (Cat#2475). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RNA binding motif protein 8A antibody (Cat#2475, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with RNA binding motif protein 8A antibody (Cat#2475, 1:1,000). Nuclei were stained blue with DAPI; RNA binding motif protein 8A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of RNA binding motif protein 8A expression in HeLa cells using RNA binding motif protein 8A antibody (Cat#2475, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RNA binding motif protein 8A.
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