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UBTF Recombinant Rabbit mAb

#5438

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Recombinant Antibody
Western blotting analysis using UBTF antibody (Cat#5438). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBTF antibody (Cat#5438, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of UBTF expression in HAP-1 cells using UBTF antibody (Cat#5438, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, UBTF.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    UBTF
  • Catalog:
    5438
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name UBTF Recombinant Rabbit mAb
Aliases UBTF; Upstream Binding Transcription Factor; UBF1; UBF; NOR-90; UBF2; Upstream Binding Transcription Factor, RNA Polymerase I; Nucleolar Transcription Factor 1; Autoantigen NOR-90;
UBF-1; 90-KDa Nucleolus Organizer Region Autoantigen; Upstream-Binding Factor 1; CONDBA
Background

Gene Name: UBTF

NCBI Gene Entry: 7343

UniProt Entry: P17480

Application Information

Molecular Weight: Predicted, 89 kDa; observed, 75-90 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB1555

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human UBTF
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using UBTF antibody (Cat#5438). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBTF antibody (Cat#5438, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of UBTF expression in HAP-1 cells using UBTF antibody (Cat#5438, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, UBTF.
Western blotting analysis using UBTF antibody (Cat#5438). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBTF antibody (Cat#5438, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of UBTF expression in HAP-1 cells using UBTF antibody (Cat#5438, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, UBTF.
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