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KO-Validated Chromobox 3 Mouse mAb

#71257

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Knockout Validated
Western blotting analysis using chromobox 3 antibody (Cat#71257). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with chromobox 3 antibody (Cat#71257, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using chromobox 3 antibody (Cat#71257). Chromobox 3 expression in wild-type (WT) and chromobox 3 (CBX3) knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with chromobox 3 antibody (Cat#71257, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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  • Gene Symbol:
    CBX3
  • Catalog:
    71257
  • Application:
    WB
  • Species Reactivity:
    H, M, R
Information
Product Name KO-Validated Chromobox 3 Mouse mAb
Aliases CBX3; Chromobox 3; HP1Hs-Gamma; Heterochromatin Protein 1 Homolog Gamma; Chromobox Protein Homolog 3; Modifier 2 Protein; HP1gamma; HECH; Chromobox Homolog 3 (HP1 Gamma Homolog, Drosophila); Chromobox Homolog 3 (Drosophila HP1 Gamma); Heterochromatin Protein HP1 Gamma; HP1 Gamma Homolog (Drosophila); Heterochromatin-Like Protein 1; Chromobox Homolog 3; HP1 Gamma Homolog; HP1-GAMMA; HP1 Gamma
Background

Gene Name: CBX3

NCBI Gene Entry: 11335

UniProt Entry: Q13185

Application Information

Molecular Weight: Predicted, 21 kDa; observed, 21 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB3825

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human HP1-gamma
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using chromobox 3 antibody (Cat#71257). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with chromobox 3 antibody (Cat#71257, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using chromobox 3 antibody (Cat#71257). Chromobox 3 expression in wild-type (WT) and chromobox 3 (CBX3) knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with chromobox 3 antibody (Cat#71257, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using chromobox 3 antibody (Cat#71257). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with chromobox 3 antibody (Cat#71257, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using chromobox 3 antibody (Cat#71257). Chromobox 3 expression in wild-type (WT) and chromobox 3 (CBX3) knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with chromobox 3 antibody (Cat#71257, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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