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Home>Product>Primary Antibody

KD-Validated CALCOCO2 Mouse mAb

#61784

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Knockdown Validated
Western blotting analysis using CALCOCO2 antibody (Cat#61784). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CALCOCO2 antibody (Cat#61784, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using CALCOCO2 antibody (Cat#61784). CALCOCO2 expression in wild-type (WT) and CALCOCO2 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 β serves as a loading control. The blot was incubated with CALCOCO2 antibody (Cat#61784, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CALCOCO2 antibody (Cat#61784). CALCOCO2 expression in wild-type (WT) and CALCOCO2 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CALCOCO2 antibody (Cat#61784, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells with CALCOCO2 antibody (Cat#61784, 1:1,000). Nuclei were stained blue with DAPI; CALCOCO2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of CALCOCO2 expression in HeLa cells using CALCOCO2 antibody (Cat#61784, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CALCOCO2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CALCOCO2
  • Catalog：
    61784
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated CALCOCO2 Mouse mAb
Aliases CALCOCO2; Calcium Binding And Coiled-Coil Domain 2; NDP52; Calcium-Binding And Coiled-Coil Domain-Containing Protein 2; Antigen Nuclear Dot 52 KDa Protein; Nuclear Domain 10 Protein NDP52; Nuclear Domain 10 Protein 52; Nuclear Dot Protein 52; MGC17318
Background

Gene Name: CALCOCO2

NCBI Gene Entry: 10241

UniProt Entry: Q13137

Application Information

Molecular Weight: Predicted, 52 kDa; observed, 52 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB3745

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human CALCOCO2
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using CALCOCO2 antibody (Cat#61784). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CALCOCO2 antibody (Cat#61784, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using CALCOCO2 antibody (Cat#61784). CALCOCO2 expression in wild-type (WT) and CALCOCO2 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 β serves as a loading control. The blot was incubated with CALCOCO2 antibody (Cat#61784, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using CALCOCO2 antibody (Cat#61784). CALCOCO2 expression in wild-type (WT) and CALCOCO2 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CALCOCO2 antibody (Cat#61784, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HeLa cells with CALCOCO2 antibody (Cat#61784, 1:1,000). Nuclei were stained blue with DAPI; CALCOCO2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of CALCOCO2 expression in HeLa cells using CALCOCO2 antibody (Cat#61784, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CALCOCO2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CALCOCO2
  • Catalog：
    61784
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using CALCOCO2 antibody (Cat#61784). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CALCOCO2 antibody (Cat#61784, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using CALCOCO2 antibody (Cat#61784). CALCOCO2 expression in wild-type (WT) and CALCOCO2 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 β serves as a loading control. The blot was incubated with CALCOCO2 antibody (Cat#61784, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CALCOCO2 antibody (Cat#61784). CALCOCO2 expression in wild-type (WT) and CALCOCO2 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CALCOCO2 antibody (Cat#61784, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells with CALCOCO2 antibody (Cat#61784, 1:1,000). Nuclei were stained blue with DAPI; CALCOCO2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of CALCOCO2 expression in HeLa cells using CALCOCO2 antibody (Cat#61784, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CALCOCO2.
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