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UBE2D3 Recombinant Rabbit mAb

#5624

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Recombinant Antibody
Western blotting analysis using UBE2D3 antibody (Cat#5624). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBE2D3 antibody (Cat#5624, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of UBE2D3 expression in C2C12 cells using UBE2D3 antibody (Cat#5624, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, UBE2D3.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    UBE2D3
  • Catalog:
    5624
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name UBE2D3 Recombinant Rabbit mAb
Aliases UBE2D3; Ubiquitin Conjugating Enzyme E2 D3; UbcH5C; Ubiquitin-Conjugating Enzyme E2D 3 (Homologous To Yeast UBC4/5); Ubiquitin-Conjugating Enzyme E2D 3 (UBC4/5 Homolog, Yeast); (E3-Independent) E2 Ubiquitin-Conjugating Enzyme D3; Ubiquitin-Conjugating Enzyme E2-17 KDa 3; Ubiquitin-Conjugating Enzyme E2(17)KB 3; Ubiquitin-Conjugating Enzyme E2 D3; E2 Ubiquitin-Conjugating Enzyme D3; Ubiquitin Carrier Protein D3; Ubiquitin-Protein Ligase D3; Ubiquitin-Conjugating Enzyme E2D 3; Ubiquitin Conjugating Enzyme E2D 3; EC 2.3.2.23; EC 2.3.2.24; EC 6.3.2.19; E2(17)KB3; UBC4/5; UBCH5C; UBC5C
Background

Gene Name: UBE2D3

NCBI Gene Entry: 7323

UniProt Entry: P61077

Application Information

Molecular Weight: Predicted, 17 kDa; observed, 17 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4575

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human UBE2D3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using UBE2D3 antibody (Cat#5624). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBE2D3 antibody (Cat#5624, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of UBE2D3 expression in C2C12 cells using UBE2D3 antibody (Cat#5624, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, UBE2D3.
Western blotting analysis using UBE2D3 antibody (Cat#5624). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBE2D3 antibody (Cat#5624, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of UBE2D3 expression in C2C12 cells using UBE2D3 antibody (Cat#5624, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, UBE2D3.
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