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PSIP1 Mouse mAb

#6468

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Western blotting analysis using PSIP1 antibody (Cat#6468). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSIP1 antibody (Cat#6468, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with PSIP1 antibody (Cat#6468, 1:1,000). Nuclei were stained blue with DAPI; PSIP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PSIP1 expression in HAP-1 cells using PSIP1 antibody (Cat#6468, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PSIP1.
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Unit price:
$69.00
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  • Gene Symbol:
    PSIP1
  • Catalog:
    6468
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name PSIP1 Mouse mAb
Aliases PSIP1; PC4 And SRSF1 Interacting Protein 1; LEDGF; DFS70; Lens Epithelium-Derived Growth Factor; PSIP2; P52; P75; PC4 And SFRS1 Interacting Protein 1; Dense Fine Speckles 70 KDa Protein; PC4 And SFRS1-Interacting Protein; CLL-Associated Antigen KW-7; PC4 And SFRS1 Interacting Protein 2; Transcriptional Coactivator P52/P75; Transcriptional Coactivator P75/P52; DFS 70; PAIP
Background

Gene Name: PSIP1

NCBI Gene Entry: 11168

UniProt Entry: O75475

Application Information

Molecular Weight: Predicted, 60 kDa; observed, 74 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9235

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen Recombinant protein of human PSIP1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:4,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PSIP1 antibody (Cat#6468). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSIP1 antibody (Cat#6468, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with PSIP1 antibody (Cat#6468, 1:1,000). Nuclei were stained blue with DAPI; PSIP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of PSIP1 expression in HAP-1 cells using PSIP1 antibody (Cat#6468, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PSIP1.
Western blotting analysis using PSIP1 antibody (Cat#6468). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSIP1 antibody (Cat#6468, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with PSIP1 antibody (Cat#6468, 1:1,000). Nuclei were stained blue with DAPI; PSIP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PSIP1 expression in HAP-1 cells using PSIP1 antibody (Cat#6468, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PSIP1.
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