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Home>Product>Primary Antibody

Histone H3 (mono methyl K9) Rabbit mAb

#R0017

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Western blotting analysis using histone H3 (mono methyl K9) antibody (Cat#R0017). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone H3 (mono methyl K9) antibody (Cat#R0017, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using histone H3 (mono methyl K9) antibody (Cat#R0017, 1:500). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of C2C12 cells with Histone H3 (mono methyl K9) antibody (Cat#R0017, 1:1,000). Nuclei were stained blue with DAPI; Histone H3 (mono methyl K9) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar: 20 μm.
Flow cytometric analysis of Histone H3 (mono methyl K9) expression in C2C12 cells using Histone H3 (mono methyl K9) antibody (Cat#R0017, 1:2,000). Green, isotype control; red, Histone H3 (mono methyl K9).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    H3C1
  • Catalog：
    R0017
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Histone H3 (mono methyl K9) Rabbit mAb
Aliases H3C1; H3 Clustered Histone 1; HIST1H3A; H3/A; H3FA; Histone Cluster 1 H3 Family Member A; H3 Histone Family, Member A; Histone Cluster 1, H3a; Histone 1, H3a; Histone H3.1; Histone H3/A; H3FC HIST1H3C; Histone H3/B; Histone H3/C; Histone H3/D; Histone H3/F; Histone H3/H; Histone H3/I; Histone; H3/J; Histone H3/K; Histone H3/L; HIST1H3B; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FL; H3FB; H3FD; H3FI; H3FH; H3FK; H3FF; H3FJ
Background

Gene Name: H3C1

NCBI Gene Entry: 8350

UniProt Entry: P68431

Application Information

Molecular Weight: Predicted, 15 kDa; observed, 17 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: S-850-153

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human Histone H3 (mono methyl K9)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:250-1:500
Note This product is for research use only.
Data
  • Western blotting analysis using histone H3 (mono methyl K9) antibody (Cat#R0017). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone H3 (mono methyl K9) antibody (Cat#R0017, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using histone H3 (mono methyl K9) antibody (Cat#R0017, 1:500). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of C2C12 cells with Histone H3 (mono methyl K9) antibody (Cat#R0017, 1:1,000). Nuclei were stained blue with DAPI; Histone H3 (mono methyl K9) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar: 20 μm.
  • Flow cytometric analysis of Histone H3 (mono methyl K9) expression in C2C12 cells using Histone H3 (mono methyl K9) antibody (Cat#R0017, 1:2,000). Green, isotype control; red, Histone H3 (mono methyl K9).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    H3C1
  • Catalog：
    R0017
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using histone H3 (mono methyl K9) antibody (Cat#R0017). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone H3 (mono methyl K9) antibody (Cat#R0017, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using histone H3 (mono methyl K9) antibody (Cat#R0017, 1:500). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of C2C12 cells with Histone H3 (mono methyl K9) antibody (Cat#R0017, 1:1,000). Nuclei were stained blue with DAPI; Histone H3 (mono methyl K9) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar: 20 μm.
Flow cytometric analysis of Histone H3 (mono methyl K9) expression in C2C12 cells using Histone H3 (mono methyl K9) antibody (Cat#R0017, 1:2,000). Green, isotype control; red, Histone H3 (mono methyl K9).
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