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Home>Product>Primary Antibody

Autophagy Related 12 Recombinant Rabbit mAb

#2989

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Recombinant Antibody
Western blotting analysis using ATG12 antibody (Cat#2989). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATG12 antibody (Cat#2989, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ATG12 expression in HepG2 cells using ATG12 antibody (Cat#2989, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ATG12.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATG12
  • Catalog：
    2989
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Autophagy Related 12 Recombinant Rabbit mAb
Aliases ATG12; Autophagy Related 12; APG12; APG12L; Ubiquitin-Like Protein ATG12; APG12 Autophagy 12-Like; ATG12 Autophagy Related 12 Homolog (S. Cerevisiae); Apg12 (Autophagy 12, S. Cerevisiae)-Like; APG12 Autophagy 12-Like (S. Cerevisiae); ATG12 Autophagy Related 12 Homolog; Apg12 (Autophagy, Yeast) Homolog; Autophagy-Related Protein 12; APG12-Like; HAPG12; FBR93
Background

Gene Name: ATG12

NCBI Gene Entry: 9140

UniProt Entry: O94817

Application Information

Molecular Weight: Predicted, 15 kDa; observed, 55 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3510

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human Apg12
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using ATG12 antibody (Cat#2989). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATG12 antibody (Cat#2989, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of ATG12 expression in HepG2 cells using ATG12 antibody (Cat#2989, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ATG12.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATG12
  • Catalog：
    2989
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ATG12 antibody (Cat#2989). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATG12 antibody (Cat#2989, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ATG12 expression in HepG2 cells using ATG12 antibody (Cat#2989, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ATG12.
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