Western blotting analysis using SEC23B antibody (Cat#5925). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SEC23B antibody (Cat#5925, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of SEC23B expression in C2C12 cells using SEC23B antibody (Cat#5925, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SEC23B.
SEC23B; SEC23 Homolog B, COPII Coat Complex Component; CDA-II; HEMPAS; CDAII; Protein Transport Protein Sec23B; SEC23-Related Protein B; HSec23B; CDAN2; Congenital Dyserythropoietic Anemia, Type II; SEC23 Homolog B, Coat Complex II Component; Sec23 (S. Cerevisiae) Homolog B; Sec23 Homolog B (S. Cerevisiae); Transport Protein SEC23B; SEC23-Like Protein B; CWS7
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human SEC23B
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using SEC23B antibody (Cat#5925). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SEC23B antibody (Cat#5925, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of SEC23B expression in C2C12 cells using SEC23B antibody (Cat#5925, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SEC23B.
Western blotting analysis using SEC23B antibody (Cat#5925). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SEC23B antibody (Cat#5925, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of SEC23B expression in C2C12 cells using SEC23B antibody (Cat#5925, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SEC23B.
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