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SERPINB2 Mouse mAb

#5040

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Western blotting analysis using SERPINB2 antibody (Cat#5040). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SERPINB2 antibody (Cat#5040, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HACAT cells with SERPINB2 antibody (Cat#5040, 1:1,000). Nuclei were stained blue with DAPI; SERPINB2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SERPINB2 expression in HaCaT cells using SERPINB2 antibody (Cat#5040, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SERPINB2.
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Specification:
Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    SERPINB2
  • Catalog:
    5040
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H
Information
Product Name SERPINB2 Mouse mAb
Aliases SERPINB2; Serpin Family B Member 2; PAI-2; HsT1201; PLANH2; PAI2; Serine (Or Cysteine) Proteinase Inhibitor, Clade B (Ovalbumin), Member 2; Serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 2; Placental Plasminogen Activator Inhibitor; Plasminogen Activator Inhibitor 2; Plasminogen-Activator Inhibitor; Monocyte Arg-Serpin; Urokinase Inhibitor; Serpin B2; Plasminogen Activator Inhibitor, Type II (Arginine-Serpin); PAI
Background

Gene Name: SERPINB2

NCBI Gene Entry: 5055

UniProt Entry: P05120

Application Information

Molecular Weight: Predicted, 47 kDa; observed, 43 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB13985

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen Recombinant protein of human SERPINB2
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:200-1:1,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using SERPINB2 antibody (Cat#5040). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SERPINB2 antibody (Cat#5040, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HACAT cells with SERPINB2 antibody (Cat#5040, 1:1,000). Nuclei were stained blue with DAPI; SERPINB2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of SERPINB2 expression in HaCaT cells using SERPINB2 antibody (Cat#5040, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SERPINB2.
Western blotting analysis using SERPINB2 antibody (Cat#5040). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SERPINB2 antibody (Cat#5040, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HACAT cells with SERPINB2 antibody (Cat#5040, 1:1,000). Nuclei were stained blue with DAPI; SERPINB2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SERPINB2 expression in HaCaT cells using SERPINB2 antibody (Cat#5040, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SERPINB2.
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