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KD-Validated Galectin 3 Rabbit pAb

#64962

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shRNA Knockdown Validated
Western blotting analysis using galectin 3 antibody (Cat#64962). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with galectin 3 antibody (Cat#64962, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using galectin 3 antibody (Cat #64962). Galectin 3 expression in wild-type (WT) and galectin 3 (LGALS3) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with galectin 3 antibody (Cat #64962, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    LGALS3
  • Catalog:
    64962
  • Application:
    WB
  • Species Reactivity:
    H, M, R
Information
Product Name KD-Validated Galectin 3 Rabbit pAb
Aliases LGALS3; Galectin 3; GALIG; Advanced Glycation End-Product Receptor 3; Lectin, Galactoside-Binding, Soluble, 3; Carbohydrate-Binding Protein 35; Galactose-Specific Lectin 3; Laminin-Binding Protein; IgE-Binding Protein; 35 KDa Lectin; Lectin L-29; Galectin-3; LGALS2; MAC-2; GALBP; MAC2; Epididymis Secretory Sperm Binding Protein; Galactoside-Binding Protein; MAC-2 Antigen; Mac-2 Antigen; CBP 35; CBP35; Gal-3; GAL3; L-31; L31
Background

Gene Name: LGALS3

NCBI Gene Entry: 3958

UniProt Entry: P17931

Application Information

Molecular Weight: Predicted, 26 kDa; observed, 31 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human Galectin 3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Note This product is for research use only.
Data
  • Western blotting analysis using galectin 3 antibody (Cat#64962). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with galectin 3 antibody (Cat#64962, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using galectin 3 antibody (Cat #64962). Galectin 3 expression in wild-type (WT) and galectin 3 (LGALS3) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with galectin 3 antibody (Cat #64962, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
Western blotting analysis using galectin 3 antibody (Cat#64962). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with galectin 3 antibody (Cat#64962, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using galectin 3 antibody (Cat #64962). Galectin 3 expression in wild-type (WT) and galectin 3 (LGALS3) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with galectin 3 antibody (Cat #64962, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
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