Western blotting analysis using histone deacetylase 9 antibody (Cat#61155). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone deacetylase 9 antibody (Cat#61155, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using histone deacetylase 9 antibody (Cat#61155). Histone deacetylase 9 expression in wild type (WT) and histone deacetylase 9 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with histone deacetylase 9 antibody (Cat#61155, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse brain using histone deacetylase 9 antibody (Cat#61155, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on frozen sections of mouse aorta using HDAC9 antibody (Cat#61155, 1:500). Nuclei were stained blue with DAPI. HDAC9 was stained red with CoraLite594. Images were taken using a confocal microscope.
The image itself is not subject to copyright protection. It was provided by a customer for educational purposes only and does not represent an endorsement by the customer of any products or services offered by the company.
Immunocytochemical staining of HepG2 cells with Histone deacetylase 9 antibody (Cat#61155, 1:1,000). Nuclei were stained blue with DAPI; Histone deacetylase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Histone deacetylase 9 expression in HepG2 cells using Histone deacetylase 9 antibody (Cat#61155, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Histone deacetylase 9.
