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Home>Product>Primary Antibody

FYN Binding Protein 1 Recombinant Rabbit mAb

#3279

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Recombinant Antibody
Western blotting analysis using FYN binding protein 1 antibody (Cat#3279). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FYN binding protein 1 antibody (Cat#3279, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9C2 cells with FYN binding protein 1 antibody (Cat#3279, 1:1,000). Nuclei were stained blue with DAPI; FYN binding protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of FYN binding protein 1 expression in H9c2 cells using FYN binding protein 1 antibody (Cat#3279, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, FYN binding protein 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FYB1
  • Catalog：
    3279
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    R

Information

Data

Information
Product Name FYN Binding Protein 1 Recombinant Rabbit mAb
Aliases FYB1; FYN Binding Protein 1; FYB-120/130; SLAP-130; ADAP; FYB; Adhesion And Degranulation Promoting Adaptor Protein; SLP-76-Associated Phosphoprotein; FYN-Binding Protein 1; FYN-T-Binding Protein; P120/P130; SLAP130; Adhesion And Degranulation-Promoting Adaptor Protein; FYN-Binding Protein (FYB-120/130); FYN Binding Protein; PRO0823; THC3
Background

Gene Name: FYB1

NCBI Gene Entry: 2533

UniProt Entry: O15117

Application Information

Molecular Weight: Predicted, 85 kDa; observed, 120 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4850

Species Reactivity: Rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human FYB
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB):1:2,500-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using FYN binding protein 1 antibody (Cat#3279). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FYN binding protein 1 antibody (Cat#3279, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of H9C2 cells with FYN binding protein 1 antibody (Cat#3279, 1:1,000). Nuclei were stained blue with DAPI; FYN binding protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of FYN binding protein 1 expression in H9c2 cells using FYN binding protein 1 antibody (Cat#3279, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, FYN binding protein 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FYB1
  • Catalog：
    3279
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    R
RELATED PRODUCTS
Western blotting analysis using FYN binding protein 1 antibody (Cat#3279). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FYN binding protein 1 antibody (Cat#3279, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9C2 cells with FYN binding protein 1 antibody (Cat#3279, 1:1,000). Nuclei were stained blue with DAPI; FYN binding protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of FYN binding protein 1 expression in H9c2 cells using FYN binding protein 1 antibody (Cat#3279, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, FYN binding protein 1.
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