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Integrin Subunit Beta 3 Recombinant Rabbit mAb

#3293

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Recombinant Antibody
Western blotting analysis using integrin subunit beta 3 antibody (Cat#3293). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with integrin subunit beta 3 antibody (Cat#3293, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Integrin subunit beta 3 antibody (Cat#3293, 1:1,000). Nuclei were stained blue with DAPI; Integrin subunit beta 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    ITGB3
  • Catalog:
    3293
  • Application:
    WB, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name Integrin Subunit Beta 3 Recombinant Rabbit mAb
Aliases ITGB3; Integrin Subunit Beta 3; GPIIIa; CD61; GP3A; Integrin, Beta 3 (Platelet Glycoprotein IIIa, Antigen CD61); Platelet Membrane Glycoprotein IIIa; Integrin Beta-3; Antigen CD61; Integrin Beta Chain, Beta 3; Platelet Glycoprotein IIIa; Integrin Beta 3; CD61 Antigen; BDPLT16; BDPLT24; BDPLT2; GT2; GT
Background

Gene Name: ITGB3

NCBI Gene Entry: 3690

UniProt Entry: P05106

Application Information

Molecular Weight: Predicted, 87 kDa; observed, 100 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4920

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen Recombinant protein of human Integrin beta 3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using integrin subunit beta 3 antibody (Cat#3293). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with integrin subunit beta 3 antibody (Cat#3293, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with Integrin subunit beta 3 antibody (Cat#3293, 1:1,000). Nuclei were stained blue with DAPI; Integrin subunit beta 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using integrin subunit beta 3 antibody (Cat#3293). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with integrin subunit beta 3 antibody (Cat#3293, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Integrin subunit beta 3 antibody (Cat#3293, 1:1,000). Nuclei were stained blue with DAPI; Integrin subunit beta 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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