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Home>Product>Primary Antibody

Splicing Factor 3B Subunit 3 Recombinant Rabbit mAb

#3329

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Recombinant Antibody
Western blotting analysis using splicing factor 3b subunit 3 antibody (Cat#3329). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with splicing factor 3b subunit 3 antibody (Cat#3329, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Splicing factor 3b subunit 3 antibody(Cat#3329, 1:1,000). Nuclei were stained blue with DAPI; Splicing factor 3b subunit 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of splicing factor 3b subunit 3 expression in HepG2 cells using splicing factor 3b subunit 3 antibody (Cat#3329, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, splicing factor 3b subunit 3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SF3B3
  • Catalog：
    3329
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Splicing Factor 3B Subunit 3 Recombinant Rabbit mAb
Aliases SF3B3; Splicing Factor 3b Subunit 3; SAP130; Spliceosome-Associated Protein 130; KIAA0017; SF3b130; RSE1; Splicing Factor 3B Subunit 3; STAF130; SAP 130; Pre-MRNA Splicing Factor SF3b, 130 KDa Subunit; Pre-MRNA-Splicing Factor SF3b 130 KDa Subunit; Splicing Factor 3b, Subunit 3, 130kDa; Splicing Factor 3b, Subunit 3, 130kD; SF3B130
Background

Gene Name: SF3B3

NCBI Gene Entry: 23450

UniProt Entry: Q15393

Application Information

Molecular Weight: Predicted, 136 kDa; observed, 136 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5085

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human SF3B3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using splicing factor 3b subunit 3 antibody (Cat#3329). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with splicing factor 3b subunit 3 antibody (Cat#3329, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with Splicing factor 3b subunit 3 antibody(Cat#3329, 1:1,000). Nuclei were stained blue with DAPI; Splicing factor 3b subunit 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of splicing factor 3b subunit 3 expression in HepG2 cells using splicing factor 3b subunit 3 antibody (Cat#3329, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, splicing factor 3b subunit 3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SF3B3
  • Catalog：
    3329
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using splicing factor 3b subunit 3 antibody (Cat#3329). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with splicing factor 3b subunit 3 antibody (Cat#3329, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Splicing factor 3b subunit 3 antibody(Cat#3329, 1:1,000). Nuclei were stained blue with DAPI; Splicing factor 3b subunit 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of splicing factor 3b subunit 3 expression in HepG2 cells using splicing factor 3b subunit 3 antibody (Cat#3329, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, splicing factor 3b subunit 3.
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