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Complement C3 Recombinant Rabbit mAb

#3371

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Recombinant Antibody
Western blotting analysis using C3 antibody (Cat#3371). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with C3 antibody (Cat#3371, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of C3 expression in HepG2 cells using C3 antibody (Cat#3371, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red,C3.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    C3
  • Catalog:
    3371
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name Complement C3 Recombinant Rabbit mAb
Aliases C3; Complement C3; CPAMD1; ARMD9; C3a; C3b; C3 And PZP-Like Alpha-2-Macroglobulin Domain-Containing Protein 1; Complement Component C3a; Complement Component C3b; Complement Component 3; C3a Anaphylatoxin; Prepro-C3; Epididymis Secretory Sperm Binding Protein Li 62p; Acylation-Stimulating Protein Cleavage Product; HEL-S-62p; AHUS5; ASP
Background

Gene Name: C3

NCBI Gene Entry: 718

UniProt Entry: P01024

Application Information

Molecular Weight: Predicted, 187 kDa; observed, 100 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5355

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human C3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using C3 antibody (Cat#3371). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with C3 antibody (Cat#3371, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of C3 expression in HepG2 cells using C3 antibody (Cat#3371, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red,C3.
Western blotting analysis using C3 antibody (Cat#3371). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with C3 antibody (Cat#3371, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of C3 expression in HepG2 cells using C3 antibody (Cat#3371, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red,C3.
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