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Home>Product>Primary Antibody

UCHL1 Recombinant Rabbit mAb

#4305

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Recombinant Antibody
Western blotting analysis using UCHL1 antibody (Cat#4305). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UCHL1 antibody (Cat#4305, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human prostatic adenocarcinoma using UCHL1 antibody (Cat#4305, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of UCHL1 expression in HAP-1 cells using UCHL1 antibody (Cat#4305, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, UCHL1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    UCHL1
  • Catalog：
    4305
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name UCHL1 Recombinant Rabbit mAb
Aliases UCHL1; Ubiquitin C-Terminal Hydrolase L1; PGP9.5; UCHL-1; Ubiquitin Carboxyl-Terminal Esterase L1 (Ubiquitin Thiolesterase); Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1; Neuron Cytoplasmic Protein 9.5; Ubiquitin Thioesterase L1; Ubiquitin Thiolesterase; PGP 9.5; Uch-L1; UCH-L1; PARK5; Epididymis Secretory Protein Li 53; Epididymis Luminal Protein 117; EC 3.4.19.12; HEL-S-53; HEL-117; SPG79A; NDGOA; PGP95; SPG79
Background

Gene Name: UCHL1

NCBI Gene Entry: 7345

UniProt Entry: P09936

Application Information

Molecular Weight: Predicted, 25 kDa; observed, 25 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10555

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human PGP9.5
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using UCHL1 antibody (Cat#4305). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UCHL1 antibody (Cat#4305, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human prostatic adenocarcinoma using UCHL1 antibody (Cat#4305, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Flow cytometric analysis of UCHL1 expression in HAP-1 cells using UCHL1 antibody (Cat#4305, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, UCHL1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    UCHL1
  • Catalog：
    4305
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using UCHL1 antibody (Cat#4305). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UCHL1 antibody (Cat#4305, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human prostatic adenocarcinoma using UCHL1 antibody (Cat#4305, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of UCHL1 expression in HAP-1 cells using UCHL1 antibody (Cat#4305, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, UCHL1.
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