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Home>Product>Primary Antibody

DEAD-Box Helicase 6 Mouse mAb

#3478

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Western blotting analysis using DEAD-box helicase 6 antibody (Cat#3478). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DEAD-box helicase 6 antibody (Cat#3478, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells with DEAD-box helicase 6 antibody (Cat#3478, 1:1,000). Nuclei were stained blue with DAPI; DEAD-box helicase 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DEAD-box helicase 6 expression in HeLa cells using DEAD-box helicase 6 antibody (Cat#3478, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DEAD-box helicase 6.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    DDX6
  • Catalog：
    3478
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name DEAD-Box Helicase 6 Mouse mAb
Aliases DDX6; DEAD-Box Helicase 6; RC; HLR2; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 6 (RNA Helicase, 54kD); DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6; Probable ATP-Dependent RNA Helicase DDX6; DEAD (Asp-Glu-Ala-Asp) Box Helicase 6; ATP-Dependent RNA Helicase P54; DEAD Box Protein 6; Oncogene RCK; Rck/P54; EC 3.6.4.13; DEAD Box-6; EC 3.6.1; RCK/P54; IDDILF; P54
Background

Gene Name: DDX6

NCBI Gene Entry: 1656

UniProt Entry: P26196

Application Information

Molecular Weight: Predicted, 54 kDa; observed, 54 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB5815

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human DDX6
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using DEAD-box helicase 6 antibody (Cat#3478). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DEAD-box helicase 6 antibody (Cat#3478, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HeLa cells with DEAD-box helicase 6 antibody (Cat#3478, 1:1,000). Nuclei were stained blue with DAPI; DEAD-box helicase 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of DEAD-box helicase 6 expression in HeLa cells using DEAD-box helicase 6 antibody (Cat#3478, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DEAD-box helicase 6.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DDX6
  • Catalog：
    3478
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using DEAD-box helicase 6 antibody (Cat#3478). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DEAD-box helicase 6 antibody (Cat#3478, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells with DEAD-box helicase 6 antibody (Cat#3478, 1:1,000). Nuclei were stained blue with DAPI; DEAD-box helicase 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DEAD-box helicase 6 expression in HeLa cells using DEAD-box helicase 6 antibody (Cat#3478, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DEAD-box helicase 6.
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