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Home>Product>Primary Antibody

ALG2 Mouse mAb

#3545

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Western blotting analysis using ALG2 antibody (Cat#3545). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALG2 antibody (Cat#3545, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with ALG2 antibody (Cat#3545, 1:1,000). Nuclei were stained blue with DAPI; ALG2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ALG2
  • Catalog：
    3545
  • Application：
    WB, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name ALG2 Mouse mAb
Aliases ALG2; ALG2 Alpha-1,3/1,6-Mannosyltransferase; HALPG2; CDGIi; NET38; CDG1I; Asparagine-Linked Glycosylation 2 Homolog (Yeast, Alpha-1,3-Mannosyltransferase); GDP-Man:Man(1)GlcNAc(2)-PP-Dol Alpha-1,3-Mannosyltransferase; GDP-Man:Man(2)GlcNAc(2)-PP-Dol Alpha-1,6-Mannosyltransferase; GDP-Man:Man(1)GlcNAc(2)-PP-Dolichol Mannosyltransferase; Asparagine-Linked Glycosylation Protein 2 Homolog; Alpha-1,3/1,6-Mannosyltransferase ALG2; FLJ14511; Asparagine-Linked Glycosylation 2, Alpha-1,3-Mannosyltransferase Homolog (S. Cerevisiae); Asparagine-Linked Glycosylation 2 Homolog (S. Cerevisiae, Alpha-1,3-Mannosyltransferase); Asparagine-Linked Glycosylation 2, Alpha-1,3-Mannosyltransferase Homolog; Alpha-1,3-Mannosyltransferase ALG2; Homolog Of Yeast ALG2; EC 2.4.1.132; EC 2.4.1.257; EC 2.4.1; CMSTA3; CMS14
Background

Gene Name: ALG2

NCBI Gene Entry: 85365

UniProt Entry: Q9H553

Application Information

Molecular Weight: Predicted, 47 kDa; observed, 49 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB6445

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human ALG2
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:2,500
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ALG2 antibody (Cat#3545). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALG2 antibody (Cat#3545, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with ALG2 antibody (Cat#3545, 1:1,000). Nuclei were stained blue with DAPI; ALG2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ALG2
  • Catalog：
    3545
  • Application：
    WB, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using ALG2 antibody (Cat#3545). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALG2 antibody (Cat#3545, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with ALG2 antibody (Cat#3545, 1:1,000). Nuclei were stained blue with DAPI; ALG2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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