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Home>Product>Primary Antibody

MAP2K3/ MAP2K6 Recombinant Rabbit mAb

#2325

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Recombinant Antibody
Western blotting analysis using MEK3/MEK6 antibody (Cat#2325). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MEK3/MEK6 antibody (Cat#2325, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with MEK3/MEK6 antibody (Cat#2325, 1:1,000). Nuclei were stained blue with DAPI; MEK3/MEK6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of MEK3/MEK6 expression in HepG2 cells using MEK3/MEK6 antibody (Cat#2325, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, MEK3/MEK6.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MAP2K3/ MAP2K6
  • Catalog：
    2325
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name MAP2K3/ MAP2K6 Recombinant Rabbit mAb
Aliases Mitogen-Activated Protein Kinase Kinase 3; MAP Kinase Kinase 3; MAPK/ERK Kinase 3; MEK3; MKK3; Dual Specificity Mitogen-Activated Protein Kinase Kinase 3; Stress-Activated Protein Kinase Kinase 2; SAPK Kinase 2; EC 2.7.12.2; SAPKK-2; MAPKK 3; MAPKK3; PRKMK3; SAPKK2; MEK 3; Dual Specificity Mitogen Activated Protein Kinase Kinase 3; MAP2K3; SKK2; Mitogen-Activated Protein Kinase Kinase 6; SAPKK3; MEK6; MKK6; MAPKK6; PRKMK6; Protein Kinase, Mitogen-Activated, Kinase 6 (MAP Kinase Kinase 6); Dual Specificity Mitogen-Activated Protein Kinase Kinase 6; Stress-Activated Protein Kinase Kinase 3; MAPK/ERK Kinase 6; SAPK Kinase 3; EC 2.7.12.2; SAPKK-3; MAPKK 6; MEK 6; MAP Kinase Kinase 6; SKK3
Background

Gene Name: MAP2K3/ MAP2K6

NCBI Gene Entry: 5606 , 5608

UniProt Entry: P46734 , P52564

Application Information

Molecular Weight: Predicted, 39,37 kDa; observed, 37 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB310

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human MEK3/MEK6
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using MEK3/MEK6 antibody (Cat#2325). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MEK3/MEK6 antibody (Cat#2325, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with MEK3/MEK6 antibody (Cat#2325, 1:1,000). Nuclei were stained blue with DAPI; MEK3/MEK6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of MEK3/MEK6 expression in HepG2 cells using MEK3/MEK6 antibody (Cat#2325, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, MEK3/MEK6.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MAP2K3/ MAP2K6
  • Catalog：
    2325
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using MEK3/MEK6 antibody (Cat#2325). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MEK3/MEK6 antibody (Cat#2325, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with MEK3/MEK6 antibody (Cat#2325, 1:1,000). Nuclei were stained blue with DAPI; MEK3/MEK6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of MEK3/MEK6 expression in HepG2 cells using MEK3/MEK6 antibody (Cat#2325, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, MEK3/MEK6.
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