Western blotting analysis using Trophoblast glycoprotein antibody (Cat#62762). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Trophoblast glycoprotein antibody (Cat#62762, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Trophoblast glycoprotein antibody (Cat#62762). Trophoblast glycoprotein expression in wild type (WT) and Trophoblast glycoprotein shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Trophoblast glycoprotein antibody (Cat#62762, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Trophoblast glycoprotein expression in HAP-1 cells using Trophoblast glycoprotein antibody (Cat#62762, 1:2,000). Green, isotype control; red, Trophoblast glycoprotein.
Validation of Trophoblast glycoprotein knockdown using flow cytometry. Wild-type(WT, Blue) and Trophoblast glycoprotein knockdown(KD, Green) HT-1080 cells were stained with Trophoblast glycoprotein antibody(Cat#62762, 1:2,000) and analyzed using BD flow cytometer.
