Western blotting analysis using NUMB antibody (Cat#69751). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NUMB antibody (Cat#69751, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NUMB antibody (Cat#69751). NUMB expression in wild type (WT) and NUMB shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with NUMB antibody (Cat#69751, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with NUMB antibody (Cat#69751, 1:1,000). Nuclei were stained blue with DAPI; NUMB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Meduim. Scale bar, 20 μm.
Flow cytometric analysis of NUMB expression in HepG2 cells using NUMB antibody (Cat#69751,1:2,000). Green, isotype control; red, NUMB.
