Western blotting analysis using keratin 8 antibody (Cat#6634). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 8 antibody (Cat#6634, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500
Note
This product is for research use only.
Data
Western blotting analysis using keratin 8 antibody (Cat#6634). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 8 antibody (Cat#6634, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using keratin 8 antibody (Cat#6634). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 8 antibody (Cat#6634, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000850s ]
SELECT * FROM `goods` WHERE `code` = '6634' LIMIT 1 [ RunTime:0.000560s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000479s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000359s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000398s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 10452 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000361s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000513s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 10452 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000323s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000423s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000271s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.057420s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5274,6547,7031,9261,7916,8952,4725,8900,8728,9136) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000742s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000572s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000229s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000546s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000276s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000374s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000446s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000285s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000235s ]
