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Home>Product>Primary Antibody

ATP5C1 Mouse mAb

#5399

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Western blotting analysis using ATP synthase F1 subunit gamma antibody (Cat#5399). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP synthase F1 subunit gamma antibody (Cat#5399, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with ATP synthase F1 subunit gamma antibody (Cat#5399, 1:1,000). Nuclei were stained blue with DAPI; ATP synthase F1 subunit gamma was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Flow cytometric analysis of ATP synthase F1 subunit gamma expression in HepG2 cells using ATP synthase F1 subunit gamma antibody (Cat#5399, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ATP synthase F1 subunit gamma.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATP5F1C
  • Catalog：
    5399
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name ATP5C1 Mouse mAb
Aliases ATP5F1C; ATP Synthase F1 Subunit Gamma; ATP5CL1; ATP5C1; ATP5C; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Gamma Polypeptide 1; ATP Synthase Subunit Gamma, Mitochondrial; F-ATPase Gamma Subunit; Mitochondrial ATP Synthase, Gamma Subunit 1; ATP Synthase Gamma Chain, Mitochondrial
Background

Gene Name: ATP5F1C

NCBI Gene Entry: 509

UniProt Entry: P36542

Application Information

Molecular Weight: Predicted, 33 kDa; observed, 33 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB1325

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human ATP5C1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:100-1:1,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ATP synthase F1 subunit gamma antibody (Cat#5399). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP synthase F1 subunit gamma antibody (Cat#5399, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with ATP synthase F1 subunit gamma antibody (Cat#5399, 1:1,000). Nuclei were stained blue with DAPI; ATP synthase F1 subunit gamma was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
  • Flow cytometric analysis of ATP synthase F1 subunit gamma expression in HepG2 cells using ATP synthase F1 subunit gamma antibody (Cat#5399, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ATP synthase F1 subunit gamma.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATP5F1C
  • Catalog：
    5399
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using ATP synthase F1 subunit gamma antibody (Cat#5399). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP synthase F1 subunit gamma antibody (Cat#5399, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with ATP synthase F1 subunit gamma antibody (Cat#5399, 1:1,000). Nuclei were stained blue with DAPI; ATP synthase F1 subunit gamma was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Flow cytometric analysis of ATP synthase F1 subunit gamma expression in HepG2 cells using ATP synthase F1 subunit gamma antibody (Cat#5399, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ATP synthase F1 subunit gamma.
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