Western blotting analysis using IKK α antibody (Cat#4197). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IKK α antibody (Cat#4197, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of IKK alpha expression in HepG2 cells using IKK alpha antibody (Cat#4197, 1:2,000). Green, isotype control; red, IKK alpha.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human IKK α
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:4,000-1:20,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using IKK α antibody (Cat#4197). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IKK α antibody (Cat#4197, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of IKK alpha expression in HepG2 cells using IKK alpha antibody (Cat#4197, 1:2,000). Green, isotype control; red, IKK alpha.
Western blotting analysis using IKK α antibody (Cat#4197). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IKK α antibody (Cat#4197, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of IKK alpha expression in HepG2 cells using IKK alpha antibody (Cat#4197, 1:2,000). Green, isotype control; red, IKK alpha.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000735s ]
SELECT * FROM `goods` WHERE `code` = '4197' LIMIT 1 [ RunTime:0.000488s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000441s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000408s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000432s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5530 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000335s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000448s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5530 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000348s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000392s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000269s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.053581s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (7576,3708,4020,3891,8118,6369,8504,6224,8013,8411) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000905s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000485s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000241s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000577s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000348s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000363s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000464s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000277s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000268s ]
