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Home>Product>Primary Antibody

IGF2BP1 Recombinant Rabbit mAb

#5209

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Recombinant Antibody
Western blotting analysis using IGF2BP1 antibody (Cat#5209). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IGF2BP1 antibody (Cat#5209, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with IGF2BP1 antibody (Cat#5209, 1:1,000). Nuclei were stained blue with DAPI; IGF2BP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of IGF2BP1 expression in HT-1080 cells using IGF2BP1 antibody (Cat#5209, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, IGF2BP1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    IGF2BP1
  • Catalog：
    5209
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name IGF2BP1 Recombinant Rabbit mAb
Aliases IGF2BP1; Insulin Like Growth Factor 2 MRNA Binding Protein 1; IMP-1; IMP1; Insulin-Like Growth Factor 2 MRNA-Binding Protein 1; Coding Region Determinant-Binding Protein; IGF-II MRNA-Binding Protein 1; IGF2 MRNA-Binding Protein 1; Zipcode-Binding Protein 1; VICKZ Family Member 1; CRD-BP; VICKZ1; CRDBP; ZBP-1; ZBP1; Insulin-Like Growth Factor 2 MRNA Binding Protein 1 DeltaN CRDBP; Insulin-Like Growth Factor 2 MRNA Binding Protein 1; IGF II MRNA Binding Protein 1
Background

Gene Name: IGF2BP1

NCBI Gene Entry: 10642

UniProt Entry: Q9NZI8

Application Information

Molecular Weight: Predicted, 63 kDa; observed, 65 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB170

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human IGF2BP1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:100-1:1,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using IGF2BP1 antibody (Cat#5209). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IGF2BP1 antibody (Cat#5209, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HT-1080 cells with IGF2BP1 antibody (Cat#5209, 1:1,000). Nuclei were stained blue with DAPI; IGF2BP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of IGF2BP1 expression in HT-1080 cells using IGF2BP1 antibody (Cat#5209, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, IGF2BP1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    IGF2BP1
  • Catalog：
    5209
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using IGF2BP1 antibody (Cat#5209). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IGF2BP1 antibody (Cat#5209, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with IGF2BP1 antibody (Cat#5209, 1:1,000). Nuclei were stained blue with DAPI; IGF2BP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of IGF2BP1 expression in HT-1080 cells using IGF2BP1 antibody (Cat#5209, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, IGF2BP1.
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