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Home>Product>Primary Antibody

Carbonyl Reductase 1 Recombinant Rabbit mAb

#3044

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Recombinant Antibody
Western blotting analysis using Carbonyl reductase 1 antibody (Cat#3044). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Carbonyl reductase 1 antibody (Cat#3044, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Carbonyl reductase 1 antibody (Cat#3044, 1:1,000). Nuclei were stained blue with DAPI; Carbonyl reductase 1 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Carbonyl reductase 1 expression in C2C12 cells using Carbonyl reductase 1 antibody (Cat#3044, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Carbonyl reductase 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CBR1
  • Catalog：
    3044
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Carbonyl Reductase 1 Recombinant Rabbit mAb
Aliases CBR1; Carbonyl Reductase 1; SDR21C1; CBR; Short Chain Dehydrogenase/Reductase Family 21C Member 1; 20-Beta-Hydroxysteroid Dehydrogenase; NADPH-Dependent Carbonyl Reductase 1; Alcohol Dehydrogenase [NAD(P)+] CBR1; Prostaglandin-E(2) 9-Reductase; Prostaglandin 9-Ketoreductase ; Carbonyl Reductase [NADPH] 1; EC 1.1.1.184; PG-9-KR; Short Chain Dehydrogenase/Reductase Family 21C, Member 1; 15-Hydroxyprostaglandin Dehydrogenase [NADP(+)]; Epididymis Secretory Sperm Binding Protein; 15-Hydroxyprostaglandin Dehydrogenase; Carbonyl Reductase (NADPH) 1; EC 1.1.1.196; EC 1.1.1.197; EC 1.1.1.189; EC 1.1.1.71; HCBR1; CRN
Background

Gene Name: CBR1

NCBI Gene Entry: 873

UniProt Entry: P16152

Application Information

Molecular Weight: Predicted, 30 kDa; observed, 30 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3785

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human CBR1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Carbonyl reductase 1 antibody (Cat#3044). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Carbonyl reductase 1 antibody (Cat#3044, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with Carbonyl reductase 1 antibody (Cat#3044, 1:1,000). Nuclei were stained blue with DAPI; Carbonyl reductase 1 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Carbonyl reductase 1 expression in C2C12 cells using Carbonyl reductase 1 antibody (Cat#3044, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Carbonyl reductase 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CBR1
  • Catalog：
    3044
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Carbonyl reductase 1 antibody (Cat#3044). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Carbonyl reductase 1 antibody (Cat#3044, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Carbonyl reductase 1 antibody (Cat#3044, 1:1,000). Nuclei were stained blue with DAPI; Carbonyl reductase 1 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Carbonyl reductase 1 expression in C2C12 cells using Carbonyl reductase 1 antibody (Cat#3044, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Carbonyl reductase 1.
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