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MALT1 Paracaspase Recombinant Rabbit mAb

#2354

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Recombinant Antibody
Western blotting analysis using MALT1 antibody (Cat#2354). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MALT1 antibody (Cat#2354, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with MALT1 antibody (Cat#2354, 1:1,000). Nuclei were stained blue with DAPI; MALT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of MALT1 expression in HepG2 cells using MALT1 antibody (Cat#2354, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, MALT1.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    MALT1
  • Catalog:
    2354
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H
Information
Product Name MALT1 Paracaspase Recombinant Rabbit mAb
Aliases MALT1 Paracaspase; PCASP1; MLT; Mucosa-Associated Lymphoid Tissue Lymphoma Translocation Protein 1; Mucosa Associated Lymphoid Tissue Lymphoma Translocation Gene 1; MALT1 Protease; MALT Lymphoma-Associated Translocation; Caspase-Like Protein; Paracaspase 1; Paracaspase-1; Paracaspase; EC 3.4.22.-; IMD12; MLT1
Background

Gene Name: MALT1

NCBI Gene Entry: 10892

UniProt Entry: Q9UDY8

Application Information

Molecular Weight: Predicted, 92 kDa; observed, 92 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB440

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human MALT1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using MALT1 antibody (Cat#2354). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MALT1 antibody (Cat#2354, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with MALT1 antibody (Cat#2354, 1:1,000). Nuclei were stained blue with DAPI; MALT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of MALT1 expression in HepG2 cells using MALT1 antibody (Cat#2354, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, MALT1.
Western blotting analysis using MALT1 antibody (Cat#2354). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MALT1 antibody (Cat#2354, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with MALT1 antibody (Cat#2354, 1:1,000). Nuclei were stained blue with DAPI; MALT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of MALT1 expression in HepG2 cells using MALT1 antibody (Cat#2354, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, MALT1.
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