Tripartite Motif Containing 28 Mouse mAb

#6580

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Western blotting analysis using tripartite motif containing 28 antibody (Cat#6580). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with tripartite motif containing 28 antibody (Cat#6580, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Immunocytochemical staining of HAP-1 cells with TRIM28 antibody (Cat#6580, 1:1,000). Nuclei were stained blue with DAPI; TRIM28 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$69.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

TRIM28
Catalog：

6580
Application：

WB, ICC
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	Tripartite Motif Containing 28 Mouse mAb
Aliases	TRIM28; Tripartite Motif Containing 28; TIF1-Beta; TIF1B; RNF96; KAP-1; KAP1; Transcription Intermediary Factor 1-Beta; RING Finger Protein 96; PPP1R157; TIF1beta; TF1B; Protein Phosphatase 1, Regulatory Subunit 157; RING-Type E3 Ubiquitin Transferase TIF1-Beta; E3 SUMO-Protein Ligase TRIM28; KRAB-Interacting Protein 1; KRAB-Associated Protein 1; Nuclear Corepressor KAP-1; KRIP-1; KRAB [Kruppel-Associated Box Domain]-Associated Protein 1; Transcriptional Intermediary Factor 1-Beta; Tripartite Motif-Containing Protein 28; Tripartite Motif-Containing 28; EC 2.3.2.27; WT7
Background	Gene Name: TRIM28 NCBI Gene Entry: 10155 UniProt Entry: Q13263
Application Information	Molecular Weight: Predicted, 89 kDa; observed, 110 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB9645 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen	Recombinant protein of human TRIM28
Isotype	Mouse IgG2a
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:2,000-1:10,000 Immunocytochemistry (ICC): 1:100-1:1,000
Note	This product is for research use only.

Data

Western blotting analysis using tripartite motif containing 28 antibody (Cat#6580). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with tripartite motif containing 28 antibody (Cat#6580, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with TRIM28 antibody (Cat#6580, 1:1,000). Nuclei were stained blue with DAPI; TRIM28 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$69.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

TRIM28
Catalog：

6580
Application：

WB, ICC
Species Reactivity：

H, M, R

Datasheet

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