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Home>Product>Primary Antibody

AP2S1 Recombinant Rabbit mAb

#3938

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Recombinant Antibody
Western blotting analysis using AP2S1 antibody (Cat#3938). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AP2S1 antibody (Cat#3938, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with AP2S1 antibody (Cat#3938, 1:1,000). Nuclei were stained blue with DAPI; AP2S1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of AP2S1 expression in C2C12 cells using AP2S1 antibody (Cat#3938, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AP2S1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AP2S1
  • Catalog：
    3938
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name AP2S1 Recombinant Rabbit mAb
Aliases AP2S1; Adaptor Related Protein Complex 2 Subunit Sigma 1; CLAPS2; FBH3; Adaptor Related Protein Complex 2 Sigma 1 Subunit; Clathrin Assembly Protein 2 Sigma Small Chain; Plasma Membrane Adaptor AP-2 17 KDa Protein; Adaptor Protein Complex AP-2 Subunit Sigma; Clathrin Coat-Associated Protein AP17; Clathrin Coat Assembly Protein AP17; AP-2 Complex Subunit Sigma; HA2 17 KDa Subunit; Sigma2-Adaptin; Sigma-2; FBHOk; AP17; HHC3; Clathrin-Associated/Assembly/Adaptor Protein, Small 2 (17kD); Adaptor-Related Protein Complex 2 Subunit Sigma; Hypocalciuric Hypercalcemia 3 (Oklahoma Type); FBHOK
Background

Gene Name: AP2S1

NCBI Gene Entry: 1175

UniProt Entry: P53680

Application Information

Molecular Weight: Predicted, 17 kDa; observed, 17 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB8780

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from AP2S1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using AP2S1 antibody (Cat#3938). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AP2S1 antibody (Cat#3938, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with AP2S1 antibody (Cat#3938, 1:1,000). Nuclei were stained blue with DAPI; AP2S1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of AP2S1 expression in C2C12 cells using AP2S1 antibody (Cat#3938, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AP2S1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AP2S1
  • Catalog：
    3938
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using AP2S1 antibody (Cat#3938). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AP2S1 antibody (Cat#3938, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with AP2S1 antibody (Cat#3938, 1:1,000). Nuclei were stained blue with DAPI; AP2S1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of AP2S1 expression in C2C12 cells using AP2S1 antibody (Cat#3938, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AP2S1.
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