Western blotting analysis using MAPRE3 antibody (Cat#2375). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAPRE3 antibody (Cat#2375, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of H9c2 cells with MAPRE3 antibody (Cat#2375, 1:1,000). Nuclei were stained blue with DAPI; MAPRE3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of MAPRE3 expression in HepG2 cells using MAPRE3 antibody (Cat#2375, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, MAPRE3.
Microtubule Associated Protein RP/EB Family Member 3; RP3; EB3; Microtubule-Associated Protein RP/EB Family Member 3; EB1 Protein Family Member 3; End-Binding Protein 3; EBF3; APC Binding Protein; EBF3-S
Western blotting analysis using MAPRE3 antibody (Cat#2375). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAPRE3 antibody (Cat#2375, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of H9c2 cells with MAPRE3 antibody (Cat#2375, 1:1,000). Nuclei were stained blue with DAPI; MAPRE3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of MAPRE3 expression in HepG2 cells using MAPRE3 antibody (Cat#2375, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, MAPRE3.
Western blotting analysis using MAPRE3 antibody (Cat#2375). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAPRE3 antibody (Cat#2375, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of H9c2 cells with MAPRE3 antibody (Cat#2375, 1:1,000). Nuclei were stained blue with DAPI; MAPRE3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of MAPRE3 expression in HepG2 cells using MAPRE3 antibody (Cat#2375, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, MAPRE3.
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