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Home>Product>Primary Antibody

KD-Validated AHSA1 Mouse mAb

#66929

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shRNA Knockdown Validated
Western blotting analysis using AHSA1 antibody (Cat#66929). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AHSA1 antibody (Cat#66929, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using AHSA1 antibody (Cat#66929). AHSA1 expression in wild type (WT) and AHSA1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AHSA1 antibody (Cat#66929, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with AHSA1 antibody (Cat#66929, 1:1,000). Nuclei were stained blue with DAPI; AHSA1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AHSA1
  • Catalog：
    66929
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated AHSA1 Mouse mAb
Aliases AHSA1; Activator Of HSP90 ATPase Activity 1; P38; AHA1; Activator Of 90 KDa Heat Shock Protein ATPase Homolog 1; C14orf3; HAha1; AHA1, Activator Of Heat Shock 90kDa Protein ATPase Homolog 1 (Yeast); AHA1, Activator Of Heat Shock 90kDa Protein ATPase Homolog 1; Chromosome 14 Open Reading Frame 3; C14ORF3; HAHA1; Aha1
Background

Gene Name: AHSA1

NCBI Gene Entry: 10598

UniProt Entry: O95433

Application Information

Molecular Weight: Predicted, 38 kDa; observed, 38 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB1990

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human AHSA1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using AHSA1 antibody (Cat#66929). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AHSA1 antibody (Cat#66929, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using AHSA1 antibody (Cat#66929). AHSA1 expression in wild type (WT) and AHSA1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AHSA1 antibody (Cat#66929, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HT-1080 cells with AHSA1 antibody (Cat#66929, 1:1,000). Nuclei were stained blue with DAPI; AHSA1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AHSA1
  • Catalog：
    66929
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using AHSA1 antibody (Cat#66929). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AHSA1 antibody (Cat#66929, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using AHSA1 antibody (Cat#66929). AHSA1 expression in wild type (WT) and AHSA1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AHSA1 antibody (Cat#66929, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with AHSA1 antibody (Cat#66929, 1:1,000). Nuclei were stained blue with DAPI; AHSA1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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