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  • Primary Antibody
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Home>Product>Primary Antibody

Tripartite Motif Containing 28 Mouse mAb

#6580

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Western blotting analysis using tripartite motif containing 28 antibody (Cat#6580). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with tripartite motif containing 28 antibody (Cat#6580, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with tripartite motif containing 28 antibody (Cat#6580, 1:1,000) . Nuclei were stained blue with DAPI; Tripartite motif containing 28 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL，100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    TRIM28
  • Catalog：
    6580
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Tripartite Motif Containing 28 Mouse mAb
Aliases TRIM28; Tripartite Motif Containing 28; TIF1-Beta; TIF1B; RNF96; KAP-1; KAP1; Transcription Intermediary Factor 1-Beta; RING Finger Protein 96; PPP1R157; TIF1beta; TF1B; Protein Phosphatase 1, Regulatory Subunit 157; RING-Type E3 Ubiquitin Transferase TIF1-Beta; E3 SUMO-Protein Ligase TRIM28; KRAB-Interacting Protein 1; KRAB-Associated Protein 1; Nuclear Corepressor KAP-1; KRIP-1; KRAB [Kruppel-Associated Box Domain]-Associated Protein 1; Transcriptional Intermediary Factor 1-Beta; Tripartite Motif-Containing Protein 28; Tripartite Motif-Containing 28; EC 2.3.2.27; WT7
Background

Gene Name: TRIM28

NCBI Gene Entry: 10155

UniProt Entry: Q13263

Application Information

Molecular Weight: Predicted, 89 kDa; observed, 110 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9645

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human TRIM28
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using tripartite motif containing 28 antibody (Cat#6580). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with tripartite motif containing 28 antibody (Cat#6580, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with tripartite motif containing 28 antibody (Cat#6580, 1:1,000) . Nuclei were stained blue with DAPI; Tripartite motif containing 28 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    TRIM28
  • Catalog：
    6580
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using tripartite motif containing 28 antibody (Cat#6580). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with tripartite motif containing 28 antibody (Cat#6580, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with tripartite motif containing 28 antibody (Cat#6580, 1:1,000) . Nuclei were stained blue with DAPI; Tripartite motif containing 28 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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    support@genuinbiotech.com

    sales@genuinbiotech.com

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    8:30 AM - 5:30 PM EST Mon-Fri

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  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
  154. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_cart.php ( 3.88 KB )
  155. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/foot.php ( 5.05 KB )
  156. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/Response.php ( 8.81 KB )
  157. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/response/Html.php ( 0.97 KB )
  158. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-trace/src/Html.php ( 4.42 KB )
  1. CONNECT:[ UseTime:0.000443s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000611s ]
  3. SELECT * FROM `goods` WHERE `code` = '6580' LIMIT 1 [ RunTime:0.002801s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000425s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000314s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.002503s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 10345 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000317s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000424s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 10345 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000282s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.002051s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000238s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.075102s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (19,9561,7903,4973,6739,7610,6441,9003,8042,5161) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000775s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000468s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000210s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000432s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000216s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000329s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000408s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000281s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000195s ]
0.133269s