Western blotting analysis using ADAMTS8 antibody (Cat#3558). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ADAMTS8 antibody (Cat#3558, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ADAMTS8 expression in C2C12 cells using ADAMTS8 antibody (Cat#3558, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ADAMTS8.
ADAMTS8; ADAM Metallopeptidase With Thrombospondin Type 1 Motif 8; ADAM-TS8; METH2; A Disintegrin-Like And Metalloprotease (Reprolysin Type) With Thrombospondin Type 1 Motif, 8; A Disintegrin And Metalloproteinase With Thrombospondin Motifs 8; FLJ41712; ADAMTS-8; METH-2; METH-8; EC 3.4.24.82; EC 3.4.24.14; EC 3.4.24.-; ADAM-TS 8; EC 3.4.24
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human ADAMTS8
Isotype
Mouse IgG2b
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:2,500-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using ADAMTS8 antibody (Cat#3558). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ADAMTS8 antibody (Cat#3558, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ADAMTS8 expression in C2C12 cells using ADAMTS8 antibody (Cat#3558, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ADAMTS8.
Western blotting analysis using ADAMTS8 antibody (Cat#3558). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ADAMTS8 antibody (Cat#3558, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ADAMTS8 expression in C2C12 cells using ADAMTS8 antibody (Cat#3558, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ADAMTS8.
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