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Home>Product>Primary Antibody

RPS6KB2 Mouse mAb

#3757

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Western blotting analysis using S6K2 antibody (Cat#3757). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with S6K2 antibody (Cat#3757, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with S6K2 antibody(Cat#3757, 1:1,000). Nuclei were stained blue with DAPI; S6K2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RPS6KB2
  • Catalog：
    3757
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name RPS6KB2 Mouse mAb
Aliases RPS6KB2; Ribosomal Protein S6 Kinase B2; STK14B; P70-BETA; P70S6Kb; S6Kbeta; S6KB; KLS; 70 KDa Ribosomal Protein S6 Kinase 2; Serine/Threonine-Protein Kinase 14B; Ribosomal Protein S6 Kinase Beta-2; P70 Ribosomal S6 Kinase Beta; S6 Kinase-Related Kinase; P70 S6 Kinase Beta; P70 S6K-Beta; EC 2.7.11.1; S6K-Beta-2; P70-S6K 2; S6K-Beta; P70 S6KB; P70S6K2; S6K2; SRK; Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 2; Ribosomal Protein S6 Kinase, 70kD, Polypeptide 2; Serine/Threonine-Protein Kinase 14 Beta; P70(S6K)-Beta; P70-Beta-1; P70-Beta-2; S6K-Beta2; EC 2.7.11; P70-Beta; S6KI(2); S6Kβ
Background

Gene Name: RPS6KB2

NCBI Gene Entry: 6199

UniProt Entry: Q9UBS0

Application Information

Molecular Weight: Predicted, 53 kDa; observed, 70 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB7230

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human S6K2
Isotype Mouse IgG1 kappa
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,500-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using S6K2 antibody (Cat#3757). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with S6K2 antibody (Cat#3757, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with S6K2 antibody(Cat#3757, 1:1,000). Nuclei were stained blue with DAPI; S6K2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RPS6KB2
  • Catalog：
    3757
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using S6K2 antibody (Cat#3757). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with S6K2 antibody (Cat#3757, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with S6K2 antibody(Cat#3757, 1:1,000). Nuclei were stained blue with DAPI; S6K2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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