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Home>Product>Primary Antibody

Fumarate Hydratase Mouse mAb

#3775

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Western blotting analysis using fumarate hydratase antibody (Cat#3775). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with fumarate hydratase antibody (Cat#3775, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Fumarate hydratase expression in HepG2 cells using Fumarate hydratase antibody (Cat#3775, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Fumarate hydratase.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FH
  • Catalog：
    3775
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name Fumarate Hydratase Mouse mAb
Aliases FH; Fumarate Hydratase; Fumarase; Fumarate Hydratase, Mitochondrial; EC 4.2.1.2; HsFH; Epididymis Secretory Sperm Binding Protein; HLRCC; MCUL1; FMRD; LRCC; MCL
Background

Gene Name: FH

NCBI Gene Entry: 2271

UniProt Entry: P07954

Application Information

Molecular Weight: Predicted, 55 kDa; observed, 45 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB8580

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human Fumarase/FH
Isotype Mouse IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using fumarate hydratase antibody (Cat#3775). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with fumarate hydratase antibody (Cat#3775, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Fumarate hydratase expression in HepG2 cells using Fumarate hydratase antibody (Cat#3775, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Fumarate hydratase.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FH
  • Catalog：
    3775
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using fumarate hydratase antibody (Cat#3775). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with fumarate hydratase antibody (Cat#3775, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Fumarate hydratase expression in HepG2 cells using Fumarate hydratase antibody (Cat#3775, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Fumarate hydratase.
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