Western blotting analysis using nudix hydrolase 19 antibody (Cat#71198). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with nudix hydrolase 19 antibody (Cat#71198, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using nudix hydrolase 19 antibody (Cat#71198). Nudix hydrolase 19 expression in wild type (WT) and nudix hydrolase 19 (NUDT19) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with nudix hydrolase 19 antibody (Cat#71198, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Nudix hydrolase 19 expression in HepG2 cells using Nudix hydrolase 19 antibody (Cat#71198, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Nudix hydrolase 19.
