Western blotting analysis using keratin 8 antibody (Cat#2922). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 8 antibody (Cat#2922, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using keratin 8 antibody (Cat#2922, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with keratin 8 antibody (Cat#2922, 1:1,000). Nuclei were stained blue with DAPI; Keratin 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Flow cytometric analysis of keratin 8 expression in HepG2 cells using keratin 8 antibody (Cat#2922, 1:2,000). Green, isotype control; red, Keratin 8.
