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Beta-Tubulin Mouse mAb

#3785

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Western blotting analysis using β-Tubulin antibody (Cat#3785). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with β-Tubulin antibody (Cat#3785, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of β-Tubulin expression in HepG2 cells using β-Tubulin antibody (Cat#3785, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, β-Tubulin.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    TUBB
  • Catalog:
    3785
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name Beta-Tubulin Mouse mAb
Aliases TUBB; Tubulin Beta Class I; OK/SW-Cl.56; Tubb5; M40; Tubulin, Beta Polypeptide; Tubulin Beta-5 Chain; Tubulin Beta Chain; MGC16435; Epididymis Secretory Sperm Binding Protein; Tubulin, Beta Class I; Class I Beta-Tubulin; Tubulin Beta-1 Chain; Beta Ib Tubulin; Beta1-Tubulin; Tubulin, Beta; CDCBM6; CSCSC1; TUBB1; TUBB5
Background

Gene Name: TUBB

NCBI Gene Entry: 203068

UniProt Entry: P07437

Application Information

Molecular Weight: Predicted, 50 kDa; observed, 50 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB7860

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human beta Tubulin
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using β-Tubulin antibody (Cat#3785). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with β-Tubulin antibody (Cat#3785, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of β-Tubulin expression in HepG2 cells using β-Tubulin antibody (Cat#3785, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, β-Tubulin.
Western blotting analysis using β-Tubulin antibody (Cat#3785). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with β-Tubulin antibody (Cat#3785, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of β-Tubulin expression in HepG2 cells using β-Tubulin antibody (Cat#3785, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, β-Tubulin.
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