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KIAA1524 Mouse mAb

#3818

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Western blotting analysis using cellular inhibitor of PP2A antibody (Cat#3818). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cellular inhibitor of PP2A antibody (Cat#3818, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Cellular inhibitor of PP2A antibody (Cat#3818, 1:500). Nuclei were stained blue with DAPI; Cellular inhibitor of PP2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Flow cytometric analysis of Cellular inhibitor of PP2A expression in HepG2 cells using Cellular inhibitor of PP2A antibody (Cat#3818, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Cellular inhibitor of PP2A.
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Unit price:
$69.00
Quantity:
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  • Gene Symbol:
    CIP2A
  • Catalog:
    3818
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H
Information
Product Name KIAA1524 Mouse mAb
Aliases CIP2A; Cellular Inhibitor Of PP2A; KIAA1524; Cell Proliferation Regulating Inhibitor Of Protein Phosphatase 2A; Cancerous Inhibitor Of Protein Phosphatase 2A; Cancerous Inhibitor Of PP2A; P90 Autoantigen; Protein CIP2A; NOCIVA; P90
Background

Gene Name: CIP2A

NCBI Gene Entry: 57650

UniProt Entry: Q8TCG1

Application Information

Molecular Weight: Predicted, 102 kDa; observed, 90 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB8025

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen Recombinant protein of human KIAA1524
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:100-1:1,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using cellular inhibitor of PP2A antibody (Cat#3818). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cellular inhibitor of PP2A antibody (Cat#3818, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with Cellular inhibitor of PP2A antibody (Cat#3818, 1:500). Nuclei were stained blue with DAPI; Cellular inhibitor of PP2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
  • Flow cytometric analysis of Cellular inhibitor of PP2A expression in HepG2 cells using Cellular inhibitor of PP2A antibody (Cat#3818, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Cellular inhibitor of PP2A.
Western blotting analysis using cellular inhibitor of PP2A antibody (Cat#3818). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cellular inhibitor of PP2A antibody (Cat#3818, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Cellular inhibitor of PP2A antibody (Cat#3818, 1:500). Nuclei were stained blue with DAPI; Cellular inhibitor of PP2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Flow cytometric analysis of Cellular inhibitor of PP2A expression in HepG2 cells using Cellular inhibitor of PP2A antibody (Cat#3818, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Cellular inhibitor of PP2A.
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