Western blotting analysis using cellular inhibitor of PP2A antibody (Cat#3818). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cellular inhibitor of PP2A antibody (Cat#3818, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Cellular inhibitor of PP2A antibody (Cat#3818, 1:500). Nuclei were stained blue with DAPI; Cellular inhibitor of PP2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Flow cytometric analysis of Cellular inhibitor of PP2A expression in HepG2 cells using Cellular inhibitor of PP2A antibody (Cat#3818, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Cellular inhibitor of PP2A.
CIP2A; Cellular Inhibitor Of PP2A; KIAA1524; Cell Proliferation Regulating Inhibitor Of Protein Phosphatase 2A; Cancerous Inhibitor Of Protein Phosphatase 2A; Cancerous Inhibitor Of PP2A; P90 Autoantigen; Protein CIP2A; NOCIVA; P90
Western blotting analysis using cellular inhibitor of PP2A antibody (Cat#3818). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cellular inhibitor of PP2A antibody (Cat#3818, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Cellular inhibitor of PP2A antibody (Cat#3818, 1:500). Nuclei were stained blue with DAPI; Cellular inhibitor of PP2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Flow cytometric analysis of Cellular inhibitor of PP2A expression in HepG2 cells using Cellular inhibitor of PP2A antibody (Cat#3818, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Cellular inhibitor of PP2A.
Western blotting analysis using cellular inhibitor of PP2A antibody (Cat#3818). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cellular inhibitor of PP2A antibody (Cat#3818, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Cellular inhibitor of PP2A antibody (Cat#3818, 1:500). Nuclei were stained blue with DAPI; Cellular inhibitor of PP2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Flow cytometric analysis of Cellular inhibitor of PP2A expression in HepG2 cells using Cellular inhibitor of PP2A antibody (Cat#3818, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Cellular inhibitor of PP2A.
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