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Home>Product>Primary Antibody

KD-Validated AK4 Mouse mAb

#66818

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shRNA Knockdown Validated
Western blotting analysis using AK4 antibody (Cat#66818). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AK4 antibody (Cat#66818, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using AK4 antibody (Cat#66818). AK4 expression in wild type (WT) and AK4 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AK4 antibody (Cat#66818, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells with AK4 antibody (Cat#66818, 1:1,000). Nuclei were stained blue with DAPI; AK4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    AK4
  • Catalog：
    66818
  • Application：
    WB, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated AK4 Mouse mAb
Aliases AK4; Adenylate Kinase 4; AK3L1; AK3; Adenylate Kinase 4, Mitochondrial; Adenylate Kinase 3-Like 1; GTP:AMP Phosphotransferase AK4, Mitochondrial; Adenylate Kinase Isoenzyme 4, Mitochondrial; Epididymis Secretory Sperm Binding Protein; Nucleoside-Triphosphate-Adenylate Kinase; Mitochondrial Adenylate Kinase-3; GTP:AMP Phosphotransferase AK4; ATP-AMP Transphosphorylase; Adenylate Kinase 3-Like; Adenylate Kinase 3; EC 2.7.4.4; EC 2.7.4.6; EC 2.7.4.3; EC 2.7.4; AK3L2; AK 4
Background

Gene Name: AK4

NCBI Gene Entry: 205

UniProt Entry: P27144

Application Information

Molecular Weight: Predicted, 25 kDa; observed, 27 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB1845

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human AK4
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using AK4 antibody (Cat#66818). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AK4 antibody (Cat#66818, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using AK4 antibody (Cat#66818). AK4 expression in wild type (WT) and AK4 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AK4 antibody (Cat#66818, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HeLa cells with AK4 antibody (Cat#66818, 1:1,000). Nuclei were stained blue with DAPI; AK4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AK4
  • Catalog：
    66818
  • Application：
    WB, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using AK4 antibody (Cat#66818). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AK4 antibody (Cat#66818, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using AK4 antibody (Cat#66818). AK4 expression in wild type (WT) and AK4 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AK4 antibody (Cat#66818, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells with AK4 antibody (Cat#66818, 1:1,000). Nuclei were stained blue with DAPI; AK4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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